Journal: Biology

Article Title: Chemical Composition, Antioxidant Capacity, and Anticancerous Effects against Human Lung Cancer Cells of a Terpenoid-Rich Fraction of Inula viscosa

doi: 10.3390/biology13090687

Figure Lengend Snippet: IVL DCM decreased the viability of A549 cells. ( A ) MTT assay was used to measure the viability of A549 and HDFn cells treated with the indicated concentrations of IVL DCM for 24, 48, or 72 h. Data represents the mean ± SEM of the percent viability of treated cells compared to vehicle-treated cells. Data are the mean of three independent experiments ( n = 3). *** p < 0.001, **** p < 0.0001. ( B ) A549 cells treated with the indicated concentrations of IVL DCM for 24 h were stained with DAPI nuclear stain. DAPI fluorescence was imaged at 20X magnification using a BioTek Cytation 5 reader. ( C ) A549 cells were treated with the indicated concentrations of IVL DCM for 24 h and then stained with crystal violet. The micrographs represent stained cells imaged at 20X magnification by light microscopy using the Invitrogen EVOS ® FL Cell Imaging System.

Article Snippet: A549 human lung adenocarcinoma cells, the most common subtype of NSCLC, which accounts for approximately 85% of lung cancer cases [ ], human normal neonatal fibroblasts (HDFn), SK-OV-3 (human ovarian cancer), MCF-7 (human breast cancer), MDA-MB-231 (human breast cancer), HepG2 (human liver cancer), and HCT116 (human colorectal cancer) cells were obtained from ATCC and cultured at 37 °C and 5% CO 2 in a humidified incubator.

Techniques: MTT Assay, Staining, Fluorescence, Light Microscopy, Imaging

Journal: Infection and Drug Resistance

Article Title: Synergism of cationic antimicrobial peptide WLBU2 with antibacterial agents against biofilms of multi-drug resistant Acinetobacter baumannii and Klebsiella pneumoniae

doi: 10.2147/IDR.S215084

Figure Lengend Snippet: Viability of Vero cells and human skin fibroblasts exposed to WLBU2. Vero cells or human skin fibroblasts were incubated with different concentrations of WLBU2 or media alone (control) for 24 hrs. Cell viability was determined using the MTT assay. Experiments were run in triplicates. Bars represent means±standard error of the mean. WLBU2 concentrations in µM correspond to 5.313, 10.625, 21.250, 42.500, 85, 170, 340, 680 µg/mL, respectively.

Article Snippet: Eukaryotic cell viability experiments utilized Vero cells (African green monkey kidney epithelial cells; ATCC CCL-81) and human skin fibroblast cells (ATCC PCS-201–012).

Techniques: Incubation, Control, MTT Assay

Journal: Infection and Drug Resistance

Article Title: Synergism of cationic antimicrobial peptide WLBU2 with antibacterial agents against biofilms of multi-drug resistant Acinetobacter baumannii and Klebsiella pneumoniae

doi: 10.2147/IDR.S215084

Figure Lengend Snippet: Viability of Vero cells and skin fibroblasts exposed to WLBU2 combined with antimicrobial agents. Vero cells or human skin fibroblasts were incubated with WLBU2 at 12.500 µM (42.5 µg/mL) or 25 µM (85 µg/mL) combined with antimicrobial agents at the indicated concentrations for 24 hrs. Cell viability was determined using the MTT assay. Experiments were run in triplicates. Bars represent means±standard error of the mean.

Article Snippet: Eukaryotic cell viability experiments utilized Vero cells (African green monkey kidney epithelial cells; ATCC CCL-81) and human skin fibroblast cells (ATCC PCS-201–012).

Techniques: Incubation, MTT Assay

Journal: International Journal of Nanomedicine

Article Title: Origanum vulgare mediated green synthesis of biocompatible gold nanoparticles simultaneously possessing plasmonic, antioxidant and antimicrobial properties

doi: 10.2147/ijn.s149819

Figure Lengend Snippet: Figure 8 Comparative cytotoxicity of 1 mL OH AuNPs and pure OVEs on fibroblasts (A) and WM35 (B) cell cultures after 24 h of exposure at a dose of 0.25, 0.5, 1.0, 2.0 and 4.0 µg/mL (mean ± SEM; n=3). Note: *p.0.05 as compared to control. Abbreviations: OH, Origanum herba; AuNP, gold nanoparticle; OVE, Origanum vulgare extract; SEM, standard error of the mean; AuNP, gold nanoparticle.

Article Snippet: For the antiproliferative effect and cell cytotoxicity assay, normal human dermal fibroblast, adult (HDFa; Thermo Fisher Scientific, Waltham, MA, USA) cell line, human radial growth phase melanoma cell line (WM35; American Type Culture Collection [ATCC], Manassas, VA, USA), fetal calf serum (FCS; HyClone), non-essential amino acids (NEAA; Sigma-Aldrich Co.), gentamicin and amphotericin (Biochrom AG, Berlin, Germany), Dulbecco’s Modified Eagle’s Medium/F12 (DMEM/F12; Sigma-Aldrich Co.), dimethyl sulfoxide (DMSO) and 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; Sigma-Aldrich Co.) have been used.

Techniques: Control

Journal: ACS Omega

Article Title: Elastomeric PU-Pluronic F‑127 Thin Films: Catalyst and Solvent Effects on Microstructure, Mechanical Properties, and Biocompatibility

doi: 10.1021/acsomega.5c11467

Figure Lengend Snippet: Biocompatibility of synthesized films. (a) Cell viability of human dermal fibroblasts (HDFs) assessed by MTT assay after 24 h incubation with film extracts. Data are mean ± SD ( n = 5). (b) Representative micrographs of HDF morphology. Viable cells show a spindle shape, while nonviable cells are rounded. Scale bar = 200 μm (10×). Sample codes: FS DMEM (Negative Control), 10% DMSO (Positive Control), CS (Commercial film extract), LA_BA (Film extract).

Article Snippet: Human dermal fibroblasts (HDFs), specifically normal human neonatal dermal fibroblasts (ATCC, Cat#PCS-201–010, Manassas, VA), were plated at a seeding density of 30% confluence and cultured in fully supplemented Dulbecco’s modified Eagle medium (FS DMEM) supplemented with 10%v/v fetal bovine serum, 100 units/mL penicillin, and 100 μg/mL streptomycin under humidified conditions at 37 °C and 5% CO 2 .

Techniques: Synthesized, MTT Assay, Incubation, Negative Control, Positive Control